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ELISA method was applied for the screening of zearalenone producing strains. The developed ELISA was as follow: 125 ㎕ of diluted solution (1 : 500) of antibody was added to each microtiter well and incubated overnight at 40℃. For direct competitive ELISA, samples and zearalenone-peroxidase conjugate were mixed in a 1 : 1 ratio, and a 100 ㎕ of aliquot was then added to antisera-coated wells. Plates were incubated for 30 minutes at 37℃, and wells washed 6 times, and 100 ㎕ of ABTS substrates was added. Plates were incubated for antother 15 minutes at 37℃, and 100 ㎕ of stopping reagent was added to the wells and absorbance was recorded at 410 ㎚ on ELISA Reader. Among 19 strains showed zearalenone-producing ability by ELISA, 3 strains (R-5, C-46, S-134) produced more than 50 ng/㎖ of zearalenone.
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